慶大黴素(Gen)ELISA快速檢測試劑盒（檢組織/肝髒/乳製品，靈敏度0.05ppb，一步法）

慶大黴素(Gen)ELISA快速檢測試劑盒

一、原理

本試劑盒采用間接競爭(zheng) ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物慶大黴素將和微孔條上預包被的偶聯抗原競爭(zheng) 抗慶大黴素抗體(ti) ，加入酶標記物後，用TMB底物顯色，樣本吸光值與(yu) 其所含殘留物慶大黴素的含量成負相關(guan) ，與(yu) 標準曲線比較即可得出相應殘留物慶大黴素的含量。

二、試劑盒特性

u  試劑盒靈敏度：       0.05ppb

u  孵育溫度    ：       25℃

u  孵育時間    ：       30min～15min

u  批內(nei) 、批間差：       <15%

u  樣本檢測下限

組織  ·····································  2.5 ppb

肝髒  ·····································  3 ppb

牛奶  ·····································  3 ppb

u  交叉反應率

慶大黴素   ······································    100%

鏈黴素       ·······································    <1%

雙氫鏈黴素 ········································· <1%

新黴素       ···········································<1%

u  樣本回收率

組織    ·········································  90%±20%

肝髒    ·········································  80%±20%

牛奶  ···········································   90%±18%

三、試劑盒組成

四、樣品前處理步驟及檢測分析步驟

  詳見產(chan) 品說明書(shu) 。

五、儲(chu) 藏條件和保質期

1、 儲(chu) 藏條件：試劑盒於(yu) 2～8℃保存，不要冷凍。

2、 保 質 期：該產(chan) 品有效期為(wei) 1年，生產(chan) 日期見包裝盒。

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